|
| Status |
Public on Sep 13, 2013 |
| Title |
NPC_p5ko_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Neural progenitor cells
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E14.5
genotype: Prmt5F/FNes
genetic background: mixed C57BL/6 X 129S1/SvlmJ
cell type: NPC
treatment: Prmt5 depletion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with beadarray in R
|
| |
|
| Submission date |
Mar 18, 2013 |
| Last update date |
Sep 13, 2013 |
| Contact name |
Julius Müller |
| Organization name |
A*Star Singapore
|
| Department |
IMCB
|
| Lab |
Ernesto Guccione
|
| Street address |
61 Biopolis Drive
|
| City |
Singapore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE45269 |
Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery (BeadChip) |
| GSE45285 |
Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery |
|
