|
| Status |
Public on Jul 09, 2013 |
| Title |
HT PTEN 6h R2 (part b) |
| Sample type |
RNA |
| |
|
| Source name |
DLBCL cellline HT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DLBCL cellline HT
treatment: PTEN cDNA
duration in hours: 6
replicate: 2
|
| Treatment protocol |
cell line HT was retroviral transduced with PTEN cDNA or empty vector, transduced cells were selected by tetracycline, PTEN cDNA expression was induced by doxycyline
|
| Growth protocol |
cell line HT was maintained in RPMI medium with 10%FBS at 37°C with 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extractet with NucleoSpin RNAII kit (illumina) according to manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
RNA was transcribed and labeld by Illumina TotalPrep RNA Amplification kit according to manufacturers protocol
|
| |
|
| Hybridization protocol |
hybridization was performed with HumanHT-12 v4 Expression Bead Chip kit (Illumina) according to manufacturers protocol
|
| Scan protocol |
scan was performed according to manufacturers protocol
|
| Description |
8839265070_F
|
| Data processing |
Data were imported on raw bead level (see supplementary data linked below) and subsequently a bead level spotfilter was applied for each microarray based on the fitted density mode for the background intensities. Afterwards bead intensities of all measured microarrays were quantile-normalized and beads were grouped by measured sequence to form beadsets. Beadsets with more than 50% of their beads excluded by the spot filter were also excluded. Further analysis was performed on gene level using median aggregation and manufacturer’s annotations.
|
| |
|
| Submission date |
Mar 26, 2013 |
| Last update date |
Jul 09, 2013 |
| Contact name |
Michael Grau |
| Organization name |
University of Münster
|
| Department |
Faculty of Medicine
|
| Lab |
Translational Oncology
|
| Street address |
Albert-Schweitzer-Campus 1
|
| City |
Münster |
| State/province |
Nordrhein-Westfalen |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE45495 |
PTEN loss defines a PI3K/AKT pathway-dependent germinal center subtype of diffuse large B-cell lymphoma |
|
