|
| Status |
Public on May 02, 2013 |
| Title |
cortex-wildtype-3 |
| Sample type |
RNA |
| |
|
| Source name |
cortex from single E12.5 mouse embryo_wildtype with floxed BAF170 allele
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: BAF170fl/fl; wild type with floxed BAF170 allele
tissue: cortex from single mouse embryo
developmental stage: E12.5
|
| Growth protocol |
BAF170fl and Emx1Cre mice were maintained on a C57BL6/J background. Animals were handled in accordance with the German Animal Protection Law and with the permission of the Bezirksregierung Braunschweig.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
2.3-2.4ug of total RNA per sample was extracted using a Qiagen RNAeasy kit.
|
| Label |
biotin
|
| Label protocol |
Nugen ovation system (performed by Cambridge Genomic Services, Cambridge, UK, certified Illumina provider).
|
| |
|
| Hybridization protocol |
Illumina Direct Hybridization Assay (performed by Cambridge Genomic Services, Cambridge, UK, certified Illumina provider)
|
| Scan protocol |
Illumina protocol using Illumina BeadArray Scanner (performed by Cambridge Genomic Services, Cambridge, UK, certified Illumina provider).
|
| Description |
wt3
|
| Data processing |
Raw data was processed using Ilumina GenomeStudio software (performed by Cambridge Genomic Services, Cambridge, UK, certified Illumina provider). Probes passing a detection p-value threshold of 0.01 in at least one sample were selected using the R package lumi (Du et al., Bioinformatics 2008). The data were then transformed using variance stabilisation (Lin et al., Nucleic Acids Research 2008) and quantile normalised.
Values are variance-stabalised and quantile-normalised expression measures computed using the R package lumi (Du et al., Bioinformatics 2008; Lin et al., Nucleic Acids Research 2008). Only detected values are shown.
|
| |
|
| Submission date |
Mar 29, 2013 |
| Last update date |
May 02, 2013 |
| Contact name |
Stephen Sansom |
| E-mail(s) |
[email protected]
|
| Organization name |
Kennedy Institute of Rheumatology
|
| Department |
NDORMS
|
| Lab |
Sansom
|
| Street address |
Roosevelt Drive
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 7FY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE45629 |
Chromatin regulation by BAF170 controls cerebral cortical size and thickness. |
|
