|
| Status |
Public on Aug 15, 2006 |
| Title |
7d dark root 1h red light, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis 1cm root tips 7d dark 1hour red light
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Landsberg erecta, 7-day-old roots from dark grown seedlings 1cm root tips illuminated with 1 hour red light (12 umol m-2 s-1)
Age: 7 day old
|
| Treatment protocol |
Seeds were sterilized with 70% ethanol 5min, two rinses 90% ethanol 10s, 4 rinses sterile water. Seeds were plated on 1/2 MS media + 1% (w/v) sucrose, 1.2% agar, pH5.6, incubated at 23 °C in the dark for 7 days. Seedlings illuminated with 1 h of 12-14 umol m-2 s-1 red light 630 nm max wavelength.
|
| Growth protocol |
Roots were grown in 23°C dark for 7 days and then transferred to 1 hour red light
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Seeds collected in RNAlater (Ambion) and roots excised for further analysis
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using GeneChip One-Cycle Target Labeling Kit (Affymetrix) from 5ug of total RNA. First strand synthesis was primed with T7dT24 oligonuclided primer containing a T7 RNA polymerase promoter seqyance on the 5'end. Second strand products were cleaned and used for IVT with biotin-labeled nucelotides.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on ATH1 Arabidopsis GeneChip Array. GeneChips were washed (Molecular Probes, OR).
|
| Scan protocol |
GeneChips were scanned using a GeneCHip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from 1cm root tips of 7day old dark grown seedlings+ 1h red light replicate 1 of 3
|
| Data processing |
MAS5
|
| |
|
| Submission date |
May 26, 2006 |
| Last update date |
May 26, 2006 |
| Contact name |
Melanie Correll |
| E-mail(s) |
[email protected]
|
| Fax |
352-392-4092
|
| URL |
http://www.agen.ufl.edu/newsite/mainpages/Faculty_pages/PDF%20Profiles%20rev/Correll_Melanie.pdf
|
| Organization name |
University of Florida
|
| Department |
Agricultural and Biological Engineering
|
| Lab |
Correll
|
| Street address |
142 Frazier Rogers Hall
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32606 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE4933 |
Expression data from 7 day old Arabidopsis roots with 1h Red light or dark |
|
