|
| Status |
Public on Apr 06, 2013 |
| Title |
ERF6-IOE line, 4h DEX-treated, biological repeat 3 |
| Sample type |
RNA |
| |
|
| Source name |
ERF6:IOE
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype: ERF6:IOE
tissue: leaf 3
age: 9DAS
treatment: 5uM dexamethasone
|
| Treatment protocol |
Plants were transferred to MS medium containing 5uM dexamethasone for 4 hours.
|
| Growth protocol |
Plants were grown on MS medium under 16 h light/ 8 h dark cycle at 22°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy plant mini kit (Qiagen), according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Agronomics protocol.
|
| Scan protocol |
Standard Agronomics protocol.
|
| Description |
ERF6:IOE_3
|
| Data processing |
Data was processed with Robust Multichip Average (RMA) (background correction, normalization, and summarization) as implemented in BioConductor (Irizarry et al., 2003a; Irizarry et al., 2003b; Gentleman et al., 2004). The Brainarray agronomics1_TAIR9_gene.cdf Chip Definition File (CDF) was used to assign probes to genes (Brainarray). The BioConductor package Limma as well as the Rank Products method was used to identify differentially expressed genes (Breitling et al., 2004; Smyth, 2004).
|
| |
|
| Submission date |
Apr 05, 2013 |
| Last update date |
Jul 30, 2013 |
| Contact name |
Marieke Dubois |
| E-mail(s) |
[email protected]
|
| Phone |
+32 (0)9 331 39 55
|
| Organization name |
VIB-PSB
|
| Department |
PSB
|
| Lab |
Systems Biology of Yield
|
| Street address |
Technologiepark 927
|
| City |
Ghent |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL14926 |
| Series (1) |
| GSE45830 |
The ETHYLENE RESPONSE FACTOR 6 Acts as a Central Regulator of Leaf Growth under Water-Limiting Conditions in Arabidopsis thaliana |
|
