|
| Status |
Public on Jun 01, 2013 |
| Title |
MGK088-1 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 TLR2-KO spleen infected with European strain (P1/7) of Streptococcus suis
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6 TLR2-KO
tissue: Spleen
gender: Female
|
| Treatment protocol |
Mice (C57BL/6 and C57BL/6 TLR2-KO) were infected for 6h with 5 x 10^7 CFU of Streptococcus suis strain P1/7.
|
| Growth protocol |
Bacterial culture was prepared by transferring 10 μl of 1/1,000 dilutions of 8-h cultures into 30 ml of THB, which was incubated for 16 h at 37°C without agitation. Stationary-phase bacteria were washed twice in PBS (pH 7.3). The bacterial pellet was then resuspended and adjusted to a concentration of 5 × 10^8 c.f.u. ml-1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was homogenized from RNAlater-stabilized spleen tissus, extracted and DNAse I treated using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Spleen S. suis P1/7 6h Rep 1
|
| Data processing |
The data were first raw-filtered in order to identify and remove probesets that were not detected and then normalised using robust spline normalization with FlexArray v1.6
|
| |
|
| Submission date |
Apr 08, 2013 |
| Last update date |
Jun 01, 2013 |
| Contact name |
Claude Lachance |
| E-mail(s) |
[email protected]
|
| Organization name |
Bio-Rad
|
| Street address |
1329 Meyerside Drive
|
| City |
Mississauga |
| State/province |
Ontario |
| ZIP/Postal code |
L5T 1C9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE45861 |
Genome wide analysis of C57BL/6 mice and C57BL/6 TLR2-KO mice infected with European strain (P1/7) of Streptococcus suis |
| GSE45863 |
Toll-like Receptor 2-independent Host Innate Immune Response Against an Epidemic Strain of Streptococcus suis that Causes a Toxic Shock-Like Syndrome in Humans |
|
