|
| Status |
Public on Apr 10, 2013 |
| Title |
Growth at 420 min |
| Sample type |
RNA |
| |
|
| Source name |
Bacillus subtilis 168 in stationary phase
|
| Organism |
Bacillus subtilis subsp. subtilis str. 168 |
| Characteristics |
growth time: 420 min
growth stage: stationary phase
optical density: O.D.600 = 2.62
|
| Treatment protocol |
Cells were centrifuged at 1,3000rpm and pellets were kept at -80OC for further extraction
|
| Growth protocol |
Bacillus subtilis 168 cells were grown in Luria-Bertani medium supplemented with glucose 0.2 % at 37°C with vigorous shaking in flasks. Overnight cultures were diluted 100-fold in fresh pre-warmed medium and samples were collected at indicated time points
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by method provided by Qiagen and Qiagen Rneasy kit was used for total RNA extraction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Bacillus subtilis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Bacillus subtilis 168 in stationary phase
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 10, 2013 |
| Last update date |
Apr 10, 2013 |
| Contact name |
CHun-Kai Yang |
| E-mail(s) |
[email protected]
|
| Organization name |
Georgia state University
|
| Department |
Biology
|
| Street address |
166 Jesse Hill Jr. Dr.
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30303 |
| Country |
USA |
| |
|
| Platform ID |
GPL343 |
| Series (1) |
| GSE45933 |
Expression data from Bacillus subtilis 168 during growth |
|
