|
| Status |
Public on Nov 06, 2013 |
| Title |
EGFRvIII-driven Glioblastoma mouse model with p53 null background, pdg044 |
| Sample type |
RNA |
| |
|
| Source name |
mouse brain
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD-1 nude
genotype/variation: EGFRvIII;p53null
disease model: Glioblastoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 3’IVT Express protocol starting with 100 ng of total RNA (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan system.
|
| Description |
Gene expression data from EGFRVIII-driven Glioblastoma mouse model
|
| Data processing |
Expression estimates were calculated using RMA in Bioconductor.
|
| |
|
| Submission date |
Apr 18, 2013 |
| Last update date |
Nov 06, 2013 |
| Contact name |
Chunxu Qu |
| E-mail(s) |
[email protected]
|
| Phone |
9015952433
|
| Organization name |
St Jude Children's Research Institute
|
| Department |
Pathology
|
| Lab |
Mullighan
|
| Street address |
262 Danny Thomas Pl
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE46190 |
Novel Oncogenic PDGFRA Mutations in Pediatric High-Grade Gliomas |
|
