|
| Status |
Public on Feb 04, 2014 |
| Title |
adult female on HFD in normoxia, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Adult female Drosophila, high-fat diet in normoxia
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole body
genotype: w1118
gender: female
age: adult, d10-12
|
| Treatment protocol |
At day 3-5, flies were put on their specific food and oxygen condition for one week.
|
| Growth protocol |
w1118 flies eclosed in 25 degree incubator and then were kept at room temperature.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was followed by RNA cleanup using RNeasy kit, according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
All samples were labeled, hyb'd and scanned by the GeneChip Microarray Core at UCSD following Affymetrix specifications.
|
| |
|
| Hybridization protocol |
All samples were labeled, hyb'd and scanned by the GeneChip Microarray Core at UCSD following Affymetrix specifications.
|
| Scan protocol |
All samples were labeled, hyb'd and scanned by the GeneChip Microarray Core at UCSD following Affymetrix specifications.
|
| Description |
Adult female Drosophila, after 1 week on high-fat diet (with 20% weight: volume coconut oil) in normoxia
|
| Data processing |
The raw data were normalized using Bioconductor Affy software (www.bioconductor.org/packages/2.0/bioc/html/affy.html) with the MAS5 algorithm, followed by analysis of the normalized data for each array spot with VAMPIRE microarray suite. A spot was considered differentially expressed between two samples when the threshold of false discovery rate (fdr) was smaller than 0.05. Significance limits were set at changes of 1.5-fold (for up-regulated genes) and 0.67-fold (for down-regulated genes).
|
| |
|
| Submission date |
Apr 24, 2013 |
| Last update date |
Feb 04, 2014 |
| Contact name |
Erilynn Heinrichsen |
| Organization name |
UC-San Diego
|
| Street address |
9500 Gilman Dr, MC0735
|
| City |
LA JOLLA |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE46358 |
Expression data from adult Drosophila females [normoxia] |
| GSE47166 |
Expression data from adult Drosophila females [normoxia and IH] |
|
