|
| Status |
Public on May 15, 2014 |
| Title |
wholeblood_woman_T-011 |
| Sample type |
RNA |
| |
|
| Source name |
did not deliver within 48 hr of hospital admission
|
| Organism |
Homo sapiens |
| Characteristics |
preterm delivery within 48 hours: No
tissue: whole blood, maternal peripheral
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral maternal blood collected using PAXgene tubes
PAXgene Blood RNA system kit was used to isolate total RNA
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to standard Affymetrix protocols (Affymetrix one-cycle kit with PNAs; Affymetrix IVT kit)
|
| |
|
| Hybridization protocol |
Hybridization was performed according to standard protocols for Affymetrix U133 plus 2.0 arrays (EukGE-WS2v5)
|
| Scan protocol |
Affymetrix GeneChip Scanner 2000
|
| Data processing |
Data were normalised using GCRMA (v.2.30.0) and differential expression was determined using Limma with multiple hypothesis testing (Benjamini and Hochberg); Bioconductor (v.1.8.3 )/R
|
| |
|
| Submission date |
Apr 30, 2013 |
| Last update date |
May 15, 2014 |
| Contact name |
Jan Heng |
| Organization name |
Lunenfeld-Tanenbaum Research Institute
|
| Street address |
25 Orde Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5T3H7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16311 |
| Series (1) |
| GSE46510 |
Whole blood gene expression profile associated with spontaneous preterm birth |
|
