|
| Status |
Public on Oct 28, 2013 |
| Title |
12H rep1 |
| Sample type |
RNA |
| |
|
| Source name |
AGS cells, 12h MPA treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: AGS
disease: gastric adenocarcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from AGS cells using a magnetic beads RNA extraction kit (Jinfiniti Biosciences, Augusta, GA). Extracted RNA was analyzed for quantity and quality using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TargetAmp-Nano labeling kit as per the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalized using RSN normalisation with lumi package in R
|
| |
|
| Submission date |
May 06, 2013 |
| Last update date |
Oct 28, 2013 |
| Contact name |
Jin-Xiong She |
| E-mail(s) |
[email protected]
|
| Phone |
706-721-3410
|
| Fax |
706-721-3688
|
| URL |
http://www.cbgm.mcg.edu/
|
| Organization name |
Medical College of Georgia
|
| Department |
Center for Biotechnology and Genomic Medicine
|
| Street address |
1120 15th Street, CA4124
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912-2400 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE46671 |
A broad array of anticancer activities of the immunosuppressive drug mycophenolic acid (MPA) is suggested by pathway analyses of gene expression and validated by proteomic and functional assays |
|
