|
| Status |
Public on Jun 09, 2013 |
| Title |
NSC_3 |
| Sample type |
RNA |
| |
|
| Source name |
mESC derived NSC
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: neural stem cellsderived from mouse embryonic stem cells
tau-redstar expression: No
cell type: neural stem cellsderived from mouse embryonic stem cells
|
| Treatment protocol |
Differentiation to neurons was performed in N2B27 for four days, followed by a replating step in Neurobasal medium supplemented with B27. Neurons were then FACS purified based on RedStar expression driven by the neuronal specific Tau promoter
|
| Growth protocol |
NSCs were derived according to monolayer protocols (Ying et al 2003) and maintained in Euromed-N medium supplemented with N2 and FGF2/EGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNA easy columns according to manufacturers protocols
|
| Label |
Biotin
|
| Label protocol |
RNA was labelled using a TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol to Illumina Mouse Ref-8 v2 BeadArrays.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
4639127051_C
|
| Data processing |
The data were normalized using quantile normalization using the Bioconductor BeadArray library in R. Differential expression was calculated using the limma library.
|
| |
|
| Submission date |
May 09, 2013 |
| Last update date |
Jun 09, 2013 |
| Contact name |
Matt Burney |
| Organization name |
King's College London
|
| Street address |
125 Coldharbour Lane
|
| City |
London |
| ZIP/Postal code |
SE5 9NU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE46791 |
An epigenetic signature of developmental potential in neural stem cells and early neurons [expression] |
| GSE46793 |
An epigenetic signature of developmental potential in neural stem cells and early neurons |
|
