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Sample GSM114055 Query DataSets for GSM114055
Status Public on Nov 21, 2006
Title large airways, smoker 010228R, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 32
Sex: M
Ethnic group: hispanic
Smoking Status: smoker, 30 pack-years
Lung: right
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray 2500.
Description large airways, non-smoker 010228R, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Dec 19, 2006
Contact name Yael Strulovici-Barel
E-mail(s) [email protected]
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL80
Series (3)
GSE5056 Airway epithelium, large airways, phenotypically normal smokers vs non-smokers, MAS5 (HuGeneFL)
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD
GSE10135 Cigarette Smoking Induces Overexpression of a Fat Depleting Gene AZGP1 in the Human Airway Epithelium

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A28102_at 1069.6 A 0.089478
AB000114_at 346.1 A 0.083592
AB000115_at 926.6 M 0.046482
AB000220_at 3211.1 P 0.00007
AB000381_s_at 21.1 A 0.794268
AB000409_at 1751.4 P 0.036569
AB000410_s_at 228 A 0.794268
AB000449_at 423.2 A 0.062929
AB000450_at 393.3 A 0.39692
AB000460_at 3516.2 P 0.000169
AB000462_at 382.1 A 0.48511
AB000464_at 1017 A 0.089478
AB000466_at 241.6 A 0.971543
AB000467_at 291.1 A 0.973889
AB000468_at 3998.8 P 0.004017
AB000584_at 6535.6 P 0.011384
AB000816_s_at 37.7 A 0.852061
AB000895_at 435.9 A 0.749204
AB000896_at 1482.6 A 0.275146
AB000897_at 497.7 A 0.425962

Total number of rows: 7129

Table truncated, full table size 197 Kbytes.




Supplementary data files not provided
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