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Sample GSM114061 Query DataSets for GSM114061
Status Public on Nov 21, 2006
Title large airways, smoker 010529R, MAS5
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics Age: 31
Sex: M
Ethnic group: white
Smoking Status: smoker, 15 pack-years
Lung: right
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HuGeneFL. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray 2500.
Description large airways, non-smoker 010529R, MAS5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 12, 2006
Last update date Dec 19, 2006
Contact name Yael Strulovici-Barel
E-mail(s) [email protected]
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL80
Series (3)
GSE5056 Airway epithelium, large airways, phenotypically normal smokers vs non-smokers, MAS5 (HuGeneFL)
GSE5060 Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD
GSE10135 Cigarette Smoking Induces Overexpression of a Fat Depleting Gene AZGP1 in the Human Airway Epithelium

Data table header descriptions
ID_REF Probe set ID
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
A28102_at 1951.2 P 0.030976
AB000114_at 592.1 A 0.48511
AB000115_at 804.8 A 0.250796
AB000220_at 1292.4 P 0.033677
AB000381_s_at 153.7 A 0.749204
AB000409_at 1843.1 A 0.216524
AB000410_s_at 236.1 A 0.904333
AB000449_at 437.7 A 0.368438
AB000450_at 972.5 A 0.175328
AB000460_at 3513 P 0.000258
AB000462_at 236.8 A 0.631562
AB000464_at 1620.6 A 0.083592
AB000466_at 920.4 A 0.724854
AB000467_at 991.9 A 0.645547
AB000468_at 2103.2 M 0.058444
AB000584_at 8014 P 0.002556
AB000816_s_at 162.2 A 0.749204
AB000895_at 620.8 A 0.52976
AB000896_at 111.3 A 0.772364
AB000897_at 601.2 A 0.440646

Total number of rows: 7129

Table truncated, full table size 197 Kbytes.




Supplementary data files not provided
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