|
| Status |
Public on May 12, 2016 |
| Title |
Illumina_control_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Illumina reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Illumina reference RNA
|
| Treatment protocol |
Treated cells were treated with 1.0ug/ml doxorubicin for two two weeks prior to harvest
|
| Growth protocol |
cells were grown in DMEM with 1mg/ml glucose, 10% fetal bovine serum, and1% penicillin, streptomycin, and fungizone solution
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed at room temperature in trizol for 30 minutes, phenol chloroform extracted, and the RNA from the upper aqueous phase was precipitated with ice cold ethanol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA (Whole-Genome Gene Expression with IntelliHyb Seal System Manual, 2006, Illumina)
|
| |
|
| Hybridization protocol |
RNAs were hybridized to Illumina WG-8 v2 arrays (Chip IDs: 1787466094 & 1714478097) according to manufacture's protocol
|
| Scan protocol |
Scanning procedures were carried out according to manufacturer’s recommendations (Illumina, Wallingford).
|
| Data processing |
Microarrays were also analyzed using GeneSpring GX (Agilent Technologies). In this analysis, expression values were normalized using a percentile shift. Raw signals were thresholded to 1.0 with a percentile target of 75. Values were then baselined to the median of all samples. All entities that were flagged as present were retained. Retained values were analyzed using ANOVA with post hoc Tukey’s HSD and Storey curve fitting for multiple testing corrections
|
| |
|
| Submission date |
May 14, 2013 |
| Last update date |
May 12, 2016 |
| Contact name |
Joshua Stevens |
| E-mail(s) |
[email protected]
|
| Phone |
3135778522
|
| Organization name |
Wayne State University School of Medicine
|
| Department |
Center for Molecular Medicine and Genetics
|
| Lab |
Heng
|
| Street address |
540 E. Canfield St.
|
| City |
Detroit |
| State/province |
MICHIGAN (MI) |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE46931 |
MDAH-041 Spontaneous Immortalization |
|
