|
| Status |
Public on May 14, 2014 |
| Title |
monocyte-sepsis-lps-4 |
| Sample type |
RNA |
| |
|
| Source name |
patient with sepsis
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: LPS
cell: monocyte
|
| Treatment protocol |
Monocytes cultured were untreated or treated ex vivo with LPS(100ng/ml) for 3h.
|
| Growth protocol |
Monocytes cultured in complete DMEM media supplemented with antibiotics (100 IU/ml penicillin and 100 µg/ml streptomycin) , in a humidified incubator at 37 °C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from monocytes using an RNAasy Mini Kit (Qiagen) as per manufacterers specifications. Extracted RNA was runned and quantified on a Bioanalyser (Agilent) for quality control and used subsequently in microarray assay.
|
| Label |
biotin
|
| Label protocol |
Total RNA samples were amplified and biotinylated using the Illumina TotalPrep RNA amplification Kit (Ambion, Austin, TX).
|
| |
|
| Hybridization protocol |
According to beadchip array manufacturer's protocol
|
| Scan protocol |
According to beadchip array manufacturer's protocol
|
| Description |
ps1.4_sepsis.LPS
|
| Data processing |
Array data were extracted at the probe level with background correction using Illumina's BeadStudio software. These raw data were then quantile normalized in R v2.15.2
|
| |
|
| Submission date |
May 15, 2013 |
| Last update date |
Jan 05, 2018 |
| Contact name |
Michael Poidinger |
| E-mail(s) |
[email protected]
|
| Phone |
+6564070528
|
| URL |
http://www.sign.a-star.edu.sg
|
| Organization name |
Singapore Immunology Network
|
| Department |
Bioinformatics
|
| Street address |
#04-06, 8A Biomedical Grove
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE46955 |
Transcriptome analysis of blood monocytes from sepsis patients |
|
