|
| Status |
Public on Jun 20, 2006 |
| Title |
Hippocampus 2003 2 month Old |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6JNIA
Age: 2 months
Gender: Male
Tissue: Whole hippocampus
|
| Biomaterial provider |
Harlan Sprague Dawley
|
| Treatment protocol |
Training of mice in the Morris water maze task was carried out between 10:00 am and 2:00 pm in four trials a day, with each
trial lasting a maximum of 120 sec with a 15-min intertrial interval. The task was performed as previously described (Malleret et al. 1999), with two training phases: 2 d with a visible platform followed by 7 d (spatial phase) with a hidden platform in the training quadrant. Probe trials (60 sec), during which the platform was removed, were performed to assess retention of the previously acquired information. Mice were killed by cervical dislocation and hippocampi were dissected out and immediately frozen in liquid nitrogen.
|
| Growth protocol |
Housed in groups and according to standard IACUC protocol. Mice received food and water ad libitum and a 12-h light/12-h dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hippocampi were homogenized, and total RNA was extracted by using TRIzol (Invitrogen Life Technologies). RNA was precipitated with isopropanol and glycogen, washed in 75% ethanol, and resuspended in RNAse free water (Ambion).
|
| Label |
Biotin
|
| Label protocol |
According to the GeneChip Expression Analysis Technical Manual (Affymetrix).
|
| |
|
| Hybridization protocol |
Fragmented amplified RNA was hybridized to Affymetrix Murine Genome U74Av2 arrays (12,486 probe sets). Hybridized arrays
were washed and stained as described in the Affymetrix signal antibody amplification protocol.
|
| Scan protocol |
Image data were acquired with
a Hewlett-Packard GeneArray scanner. Signal values were generated by using the Affymetrix Microarray Suite 5.0 software.
|
| Description |
(No additional Information)
|
| Data processing |
MAS 5.0, Target value 250
|
| |
|
| Submission date |
Jun 14, 2006 |
| Last update date |
Jun 19, 2006 |
| Contact name |
Paul Pavlidis |
| E-mail(s) |
[email protected]
|
| Organization name |
University of British Columbia
|
| Department |
Centre for High-Throughput Biology / Psychiatry
|
| Street address |
177 Michael Smith Laboratories 2185 East Mall
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5078 |
Hippocampal transcript profile in young and middle-aged mice |
|
