|
| Status |
Public on May 21, 2013 |
| Title |
Wildtype-replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type stage 13 mutant embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: dechorionated embryo
age: stage 13
genotype: w118
|
| Treatment protocol |
Embryos were dechorionated, transferred to PBS, and then staged and genotyped under a GFP Stereoscope. fra[3]/fra[3] homozygotes were selected on the basis of their lack of GFP expression.
|
| Growth protocol |
Eggs laid by fra[3]/CyOGFP parents were collected for one hour in egg-laying chambers, and then aged on molasses plates at room temperature until they reached stage 13.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Trizol (Invitrogen) based on the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA according to the standard Affymetrix protocol (GeneChip Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 in a GeneChip Hybridization Oven 640 according to the manufacturer's instructions. The GeneChips were washed and stained in an Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned with a GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data for wildtype stage 13 Drosophila embryos
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
May 20, 2013 |
| Last update date |
May 21, 2013 |
| Contact name |
Molly Duman Scheel |
| Organization name |
Indiana University School of Medicine
|
| Street address |
1234 Notre Dame Ave.
|
| City |
South Bend |
| State/province |
IN |
| ZIP/Postal code |
46617 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE47112 |
Gene expression profile of frazzled (fra) mutant Drosophila embryos |
|
