|
| Status |
Public on Aug 30, 2006 |
| Title |
XZ_colWhole_HMBDL_04_F0116_06242005.CEL |
| Sample type |
genomic |
| |
|
| Source name |
whole palnt tissue w/o root, 40 days
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Depleted for CG-methylated DNA
|
| Treatment protocol |
Untreated
|
| Growth protocol |
Plants were grown on soil under continuous illumination for 40 days at 25 degrees C
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was isolated from whole plant tissues using DNAeasy kit following instructions from the manufacturer (QIAGEN) instruction
|
| Label |
biotin
|
| Label protocol |
The genomic DNA in the samples was 3'-end-labelled with dd-UTP (Roche) by the terminal transferase (TdT, Roche) reaction for 1 h at 37 degrees C.
|
| |
|
| Hybridization protocol |
Whole Genome Tiling Array was hybridized for 16 hrs, washed and stained with a streptavidin-phycoerythrin conjugate on the Affymetrix Fluidics Station 400 according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned on Affymetrix scanner G7
|
| Description |
Depleted for CG-methylated DNA
|
| Data processing |
Gene chip data files were processed using TileMap and KS method.
|
| |
|
| Submission date |
Jun 15, 2006 |
| Last update date |
Aug 25, 2006 |
| Contact name |
Steve E Jacobsen |
| E-mail(s) |
[email protected]
|
| Phone |
(310) 825-0182
|
| URL |
http://www.mcdb.ucla.edu/Research/Jacobsen/index.html
|
| Organization name |
University of California, Los Angeles
|
| Department |
Molecular Cell and Developmental Biology
|
| Lab |
Jacobsen lab
|
| Street address |
621 Charles E. Young Dr. South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1606 |
| Country |
USA |
| |
|
| Platform ID |
GPL1979 |
| Series (1) |
| GSE5094 |
High Resolution Mapping and Functional Analysis of the Methylome in Arabidopsis (mCIP, MBD) |
|
