|
| Status |
Public on May 29, 2013 |
| Title |
LNCaP ERG siLEF1 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
genotype/variation: ERG overexpression and LEF1 knockdown
|
| Growth protocol |
LNCap cell line was maintained in RPMI1640 supplemented with 10%FBS, VCaP cell line was maintained in DMEM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. The quantity and quality of the isolated RNAs was determined using ND-1000 Spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
The arrays were processed at Cleveland Clinic according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The arrays were processed at Cleveland Clinic according to manufacturer’s protocol.
|
| Scan protocol |
The arrays were processed at Cleveland Clinic according to manufacturer’s protocol.
|
| Description |
Replicate 1
|
| Data processing |
The bead-level data were preprocessed using GenomeStudio (Illumina) and the expression data was analyzed using the beadarray package in Bioconductor, quantile normalized using the beadarray package.
|
| |
|
| Submission date |
May 28, 2013 |
| Last update date |
May 29, 2013 |
| Contact name |
Longtao WU |
| E-mail(s) |
[email protected]
|
| Phone |
312-503-3441
|
| Fax |
312-503-0189
|
| Organization name |
Northwestern University
|
| Department |
Hematology/Oncology
|
| Lab |
Jindan Yu Lab
|
| Street address |
303 E Superior St.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE47423 |
ERG is a critical regulator of Wnt/LEF1 signaling in prostate cancer |
|
