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Status
Public on Dec 31, 2013
Title
IL13_plastic_rep3
Sample type
RNA
Channel 1 Source name
IMR90, plastic, IL13
Organism
Homo sapiens Characteristics
cell line: IMR90
Treatment protocol
Cells were treated for 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), or TNFa (10 ng/ml).
Growth protocol
IMR-90 cells (ATCC, Manassas, VA; Catalog # CCL-186) were cultured in DMEM medium supplemented with 10% FBS (Sigma, St. Louis, MO; Catalog # F2442) and Penicillin/Streptomycin (Invitrogen, Carlsbad, CA; Catalog # 15140) on a bed of growth factor reduced Matrigel (GFR Matrigel, BD Biosciences, Bedford, MA; Catalog No. 354230) or plastic.
Extracted molecule
total RNA
Extraction protocol
RNeasy Mini Kit (Qiagen).
Label
Cy5
Label protocol
Biotinylated cRNA were prepared according to the standard Agilent protocol.
Channel 2 Source name
Stratagene Universal Human Reference RNA
Organism
Homo sapiens Characteristics
sample type: reference
Extracted molecule
total RNA
Extraction protocol
Stratagene Universal Human Reference RNA (catalog number 740000).
Label
Cy3
Label protocol
Biotinylated cRNA were prepared according to the standard Agilent protocol.
Hybridization protocol
cRNA was hybridized on Agilent-014850 Whole Human Genome Microarray G4112F.
Scan protocol
Chips were scanned using the Agilent Surescan Microarray Scanner.
Description
SAM630170
Data processing
The data were processed with Agilent Feature Extraction software, version 10.7.3.1, using default settings, including linear and Lowess normalization.
Submission date
May 30, 2013
Last update date
Dec 31, 2013
Contact name
Alex Abbas
E-mail(s)
[email protected] Organization name
Genentech, Inc.
Department
Bioinformatics
Street address
1 DNA Way
City
South San Francisco
State/province
CA
ZIP/Postal code
94080
Country
USA
Platform ID
GPL6480 Series (1)
GSE47542 IMR90 lung fibroblasts treated with IL-4 or IL-13
Data table header descriptions ID_REF VALUE Agilent log2 ratio, test channel (ch1)/reference channel (ch2).
Data table ID_REF VALUE A_23_P100001
-4.1535
A_23_P100011
0.46493
A_23_P100022
-1.569
A_23_P100056
-1.677
A_23_P100074
1.5878
A_23_P100092
0.59356
A_23_P100103
0.96323
A_23_P100111
0.76434
A_23_P100127
0.54424
A_23_P100133
-0.016093
A_23_P100141
0.40243
A_23_P100156
0.3459
A_23_P100177
-1.7811
A_23_P100189
0.064631
A_23_P100196
-1.0073
A_23_P100203
0.072078
A_23_P100220
-3.4919
A_23_P100240
-3.6308
A_23_P10025
-4.8172
A_23_P100263
0.7421
41107 808 Kbytes .Supplementary file Size Download File type/resource GSM1151949_Agilent_251485054012_S01_GE2_107_Sep09_1_4.txt.gz
4.4 Mb
(ftp) (http) TXT
Processed data included within Sample table
