|
| Status |
Public on Jun 01, 2014 |
| Title |
11310 - no IFN-gamma - mAdbID:120210 |
| Sample type |
RNA |
| |
|
| Source name |
11310 - no IFN-gamma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: monocyte-derived dendritic cells
individual: 11310
|
| Treatment protocol |
Agent: GM-CSF and IL-4
Treatment dose: GM-CSF (800 IU/mL) and IL-4 (1000 IU/mL)
Treatment time: 6 days
In-vitro treatment: Elutriated monocytes were prepared from 20-80 year old healthy donors. Elutriated monocytes were plated onto 24 well plates and differentiated into moDCs with RPMI-1640 (+glutamine; Invitrogen) supplemented with 10% heat-inactivated FCS, 0.5% HEPES, 1% Penicillin/Streptomycin, and 0.01% gentamycin, in the presence of GM-CSF (800 IU/mL) and IL-4 (1000 IU/mL) for 6 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sample Extraction Protocol
Other: Total RNA was isolated using the RNeasy kit (Qiagen) and was amplified using the Ambion WT expression kit (Invitrogen) according to the manual.
|
| Label |
biotin
|
| Label protocol |
Affymetrix Terminal Labeling Kit
Other: Affymetrix Terminal Labeling Kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Affymetrix Hybrization Protocol
Other: Terminally labeled cDNA were hybridized to the Human GeneChip 1.0 ST array (Affymetrix) according to the manufacturers' instructions.
|
| Scan protocol |
Affymetrix Scanning Protocol
Other: Arrays were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
mAdb experiment ID: 120210
|
| Data processing |
Affymetrix RMA Data Processing
Calculation Method: After Robust Multichip Average (RMA) normalization, differentially expressed genes were identified by One-Way Repeated Measures ANOVA (p<0.01) corrected by Benjamini-Hochberg's False Discovery Rate method (p<0.05) and this gene list was further filtered for between-group alpha levels of p<0.01, p<0.005, and p<0.001 for genes differentially expressed from moDCs differentiated with GM-CSF and IL-4 or GM-CSF, IL-4 and 1 IU of IFN-gamma. The statistically significant gene expression changes were functionally analyzed using Ingenuity Pathways Analysis (Ingenuity Systems) software.
|
| |
|
| Submission date |
Jun 04, 2013 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Francesco Maria Marincola |
| E-mail(s) |
[email protected]
|
| Phone |
301-793-8210
|
| Organization name |
Sidra Medical and Research Center
|
| Street address |
Al Nasr Tower, AL Corniche Street, PO Box 26999
|
| City |
Doha |
| ZIP/Postal code |
PO Box 26999 |
| Country |
Qatar |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE47621 |
Interferon-gamma critically determines dendritic cell function |
|
| Relations |
| Reanalyzed by |
GSE86357 |
