|
| Status |
Public on Jun 01, 2014 |
| Title |
Restricted_48h |
| Sample type |
RNA |
| |
|
| Source name |
Restricted food, 48 hours after switch
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head and thorax
genotype: Canton-S
Sex: females
age: 40 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total head and thorax RNA was isolated from at least 75 females using Trizol (Invitrogen) and further purified using RNeasy columns (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Preparation of biotynilated cRNA and hybridization to the Drosophila2 array were performed as described by the manufacturer (Affymetrix).
|
| |
|
| Hybridization protocol |
Preparation of biotynilated cRNA and hybridization to the Drosophila2 array were performed as described by the manufacturer (Affymetrix).
|
| Scan protocol |
Stained arrays were scanned using an Affymetrix 3000 G7 scanner. Data acquisition was carried out at Brown University.
|
| Description |
Gene expression data from 40d. old female flies passed in restricted food
|
| Data processing |
The data was PVAC filtered, quantile normalized and summarized using Robust Multichip Analysis (RMA) to obtain expression scores in the log2 scale.
|
| |
|
| Submission date |
Jun 04, 2013 |
| Last update date |
Jun 01, 2014 |
| Contact name |
M. Pilar Gil |
| Organization name |
Brown University
|
| Street address |
75 Waterman St
|
| City |
Providence |
| State/province |
Rhode Island |
| ZIP/Postal code |
02906 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE47631 |
Dietary switch reveals fast coordinated gene expression changes in Drosophila melanogaster. |
|
