|
| Status |
Public on Jun 08, 2009 |
| Title |
Seedling_Control_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
7-day-old light-grown rice seedlings
|
| Organism |
Oryza sativa |
| Characteristics |
Subspecies: indica
Variety: IR64
Tissue: light-grown seedlings
Stage: 7-day-old
|
| Treatment protocol |
seedlings were mock-treated with dimethyl sulfoxide (final concentration 0.1%) for 1h and 3h and pooled together in equal amount before RNA isolation
|
| Growth protocol |
Seedlings were grown on cotton saturated with reverse-osmosis water at 28 degree centigrade in a culture room with a daily photoperiodic cycle of 14h light and 10h dark
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol reagent
|
| Label |
Biotin
|
| |
|
| Description |
Rice seedlings were mock-treated for 1h and 3h to serve as control and equal amount was pooled before RNA isolation. The high-quality total RNA (260/280 ratio between 1.9-2.1 and 260/230 ratio >2.0) was used for microarray analysis
|
| Data processing |
Signal intensity was calculated by GCOS1.4 under default parameters
|
| |
|
| Submission date |
Jun 27, 2006 |
| Last update date |
Jun 08, 2009 |
| Contact name |
Mukesh Jain |
| Phone |
91-11-26704686
|
| Organization name |
Jawaharlal Nehru University
|
| Street address |
New Mehrauli Road
|
| City |
New Delhi |
| State/province |
New Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE5167 |
Rice seedling hormone treatment |
|
