|
| Status |
Public on Jun 28, 2007 |
| Title |
Sun187_BAV13 group |
| Sample type |
RNA |
| |
|
| Source name |
aorta
|
| Organism |
Homo sapiens |
| Characteristics |
aortic aneurysm
|
| Treatment protocol |
Tissue from ascending aortic aneurysms from patients undergoing ascending aortic surgery were collected. A portion of the tissue was snap frozen in liquid nitrogen and stored at –80oC until RNA extraction.
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from aortic tissue was extracted using Mini to Midi total RNA extraction kit (Invitrogen, Carlsbad, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000 according to the manufacturer's protocol.
|
| Description |
Gene expression data from aneurismal tissues obtained from patients with bicuspid valves.
|
| Data processing |
Data analysis was performed using a linear model. The probe level data were normalized using Fastlo (Ballman et al. Bioinformatics 2004; 20:2778–2786) a modified version of cyclic loess normalization (Chu et al. Math Bio 2002;176: 35-51).
|
| |
|
| Submission date |
Jun 28, 2006 |
| Last update date |
Jun 28, 2006 |
| Contact name |
Ramanath Majumdar |
| E-mail(s) |
[email protected]
|
| Phone |
507-266-4995
|
| Organization name |
Mayo Clinic
|
| Street address |
200 1st St, SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE5180 |
Gene expression in aortic aneurysms associated with tricuspid and bicuspid valves |
|
