|
| Status |
Public on Jul 26, 2013 |
| Title |
ovoD mutant_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ovoD mutants replicate 2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: female
age: 15-days-old
genotype/variation: ovoD mutant
|
| Growth protocol |
Adults from each genotype were aged to 14 days upon standard media in demography cages (125 females per cage, four cages per genotype).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each genotype, total RNA was isolated from 25 whole females with Trizol (Invitrogen) and purified upon RNeasy columns (QIAGEN). Using the Affymetrix One Cycle DNA system, 5 ug total RNA was converted to cDNA.
|
| Label |
biotin
|
| Label protocol |
Double stranded cDNA was amplified overnight at 37°C with the Affymetric IVT labeling protocol to produce biotin labeled cRNA.
|
| |
|
| Hybridization protocol |
1.5 ug of spin column cleaned cRNA was fragmented by metal inducing hydrolysis, and 1.0 ug of this product was hybridized to arrays overnight at 45°C, 60 rpm. Hybridization, washing and staining were completed on an Affymetrix 450 Fluidics Station
|
| Scan protocol |
scan was done on an Affymetrix 3000 G7
|
| Description |
Gene expression data from 15-day-old ovoD female flies.
|
| Data processing |
Quantile normalized data were processed with GCRMA [63] to generate log2 scaled expression values. Probesets with mean expression in the first quartile within a genotype were excluded. Two sided t-tests were used to assess differential expression with individual p < 0.05 and fold change at least 1.5. Probsets were collapsed to genes after differential expression statistics selection.
|
| |
|
| Submission date |
Jun 20, 2013 |
| Last update date |
Jul 28, 2013 |
| Contact name |
Hua Bai |
| E-mail(s) |
[email protected]
|
| Organization name |
Brown University
|
| Department |
EEB
|
| Lab |
Marc Tatar Lab
|
| Street address |
34 Olive St, BMC502
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE48145 |
Juvenile hormone regulation of Drosophila aging |
|
