|
| Status |
Public on Jan 07, 2014 |
| Title |
Breast tissue_Pt_119 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Normal breast tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Breast
her2: Normal
er: Positive
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from cancerous and matching normal breast tissues from the same subject (n=23) by using a QIAamp DNA mini kit (Qiagen, Valencia, CA).
|
| Label |
Cy5
|
| Label protocol |
Cy3 or Cy5 labeled DNA were prepared by Agilent Genomic DNA Enzymatic Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
Breast cancer tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Breast
her2: Normal
er: Positive
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from cancerous and matching normal breast tissues from the same subject (n=23) by using a QIAamp DNA mini kit (Qiagen, Valencia, CA).
|
| Label |
Cy3
|
| Label protocol |
Cy3 or Cy5 labeled DNA were prepared by Agilent Genomic DNA Enzymatic Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
DNA were hybridized according to Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis, V6.0.
|
| Scan protocol |
Scanned on an GenePix 4000B.
|
| Description |
Array comparative genomic hybridization between normal and cancer breast tissues without chemotherapy.
|
| Data processing |
GenePix Pro 6.0.1.12 was used for background subtraction and Linear Normalization Using Rank Consistent Probes.
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
Jan 07, 2014 |
| Contact name |
Chi-Cheng Huang |
| E-mail(s) |
[email protected]
|
| Organization name |
Cathay General Hospital SiJhih
|
| Department |
Department of Medical Education and Research
|
| Street address |
No.2, Lane 59, Jiancheng Rd. Xizhi Dist.
|
| City |
New Taipei City |
| ZIP/Postal code |
22174 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL9075 |
| Series (2) |
| GSE48297 |
Concurrent Gene Signatures for Han Chinese Breast Cancers [Agilent] |
| GSE48391 |
Concurrent Gene Signatures for Han Chinese Breast Cancers |
|
