|
| Status |
Public on Dec 23, 2014 |
| Title |
Tsetse_fly_midgut-Control-sample2 |
| Sample type |
RNA |
| |
|
| Source name |
Tsetse_fly_midgut-Control
|
| Organism |
Sodalis glossinidius |
| Characteristics |
status: Control
timepoint: 10 days
treatment: non infective blood meal
source gender: female fly
source tissue: Tsetse fly midgut
|
| Treatment protocol |
Flies were exposed to Trypanosoma brucei gambiense infected mices.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from fly midgut by TRIzol (Invitrogen) according to the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cDNA was prepared from 5µg RNA using the Chipshot Direct labeling and Clean-Up System kit (Promega) according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
600 ng of Cy3-labelled cRNA (specific activity > 6 pmol Cy3/ug cRNA) was added to 250 ml of 2x Agilent hybridization buffer and hybridized to Agilent Sodalis glossinidius custom Oligo Microarrays (earray Design ID = 041518; earray Slide Layout = IS-15744-8-V1) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 8x15k array slides.
|
| Description |
Total RNA Glossina palpalis gambiensis, 10 days after uninfected bloodmeal, sample 2
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.5.1.1 (Agilent) using default parameters (protocol GE1_107_Sep09GE1_107_Sep09 and Grid: 041518_D_F_20120607) to obtain background subtracted and spatially detrended Processed Signal intensities. All data were normalized by quantile normalization.
|
| |
|
| Submission date |
Jun 27, 2013 |
| Last update date |
Dec 23, 2014 |
| Contact name |
Anne Geiger |
| E-mail(s) |
[email protected]
|
| Organization name |
UMR 177 IRD-CIRAD
|
| Street address |
Laboratoire de Recherches et de Coordination sur les Trypanosomoses TA,A-17 / G Campus international de Baillarguet
|
| City |
Montpellier |
| ZIP/Postal code |
34000 |
| Country |
France |
| |
|
| Platform ID |
GPL17347 |
| Series (1) |
| GSE48360 |
Expression of Sodalis glossinidius genes derived from self-cleared and control Glossina palpalis gambiensis flies |
|
