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Status |
Public on Jan 07, 2014 |
Title |
Breast cancer_142T |
Sample type |
RNA |
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|
Source name |
Breast cancer tumor tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: Breast predicted risk: high hu306 classification: Normal breast-like her2: over-expression er: negative survival time (year): 1.25 event: disease-free status: alive
|
Treatment protocol |
The frozen bulk tumor samples were confirmed to contain at least 90% viable tumor by area on histologic thin sections.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from frozen specimens using TRIzol® reagent (Invitrogen, Carlsbad, CA). Purification of RNA was performed using RNeasy® mini kits (Qiagen, Valencia, CA) according to the manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA.
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Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45 °C on GeneChip® Human Genome U133 Plus 2.0 expression arrays (Affymetrix, Inc., Santa Clara, CA).
|
Scan protocol |
GeneChips were scanned using a GeneChip Scanner 3000.
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Description |
Gene expression data from breast cancer tissues without chemotherapy.
|
Data processing |
Raw gene expression values were obtained using Affymetrix GeneChip software, summarized by Robust Multichip Average (RMA) algorithm and further normalized by quantile normalization.
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Submission date |
Jun 28, 2013 |
Last update date |
Feb 28, 2014 |
Contact name |
Chi-Cheng Huang |
E-mail(s) |
[email protected]
|
Organization name |
Cathay General Hospital SiJhih
|
Department |
Department of Medical Education and Research
|
Street address |
No.2, Lane 59, Jiancheng Rd. Xizhi Dist.
|
City |
New Taipei City |
ZIP/Postal code |
22174 |
Country |
Taiwan |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE48390 |
Concurrent Gene Signatures for Han Chinese Breast Cancers [Affymetrix] |
GSE48391 |
Concurrent Gene Signatures for Han Chinese Breast Cancers |
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