|
| Status |
Public on Jan 07, 2014 |
| Title |
Breast cancer_BH0354-2 |
| Sample type |
RNA |
| |
|
| Source name |
Breast cancer tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Breast
predicted risk: high
hu306 classification: HER2-enriched
her2: over-expression
er: negative
survival time (year): 4.17
event: disease-free
status: alive
|
| Treatment protocol |
The frozen bulk tumor samples were confirmed to contain at least 90% viable tumor by area on histologic thin sections.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen specimens using TRIzol® reagent (Invitrogen, Carlsbad, CA). Purification of RNA was performed using RNeasy® mini kits (Qiagen, Valencia, CA) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45 °C on GeneChip® Human Genome U133 Plus 2.0 expression arrays (Affymetrix, Inc., Santa Clara, CA).
|
| Scan protocol |
GeneChips were scanned using a GeneChip Scanner 3000.
|
| Description |
Gene expression data from breast cancer tissues without chemotherapy.
|
| Data processing |
Raw gene expression values were obtained using Affymetrix GeneChip software, summarized by Robust Multichip Average (RMA) algorithm and further normalized by quantile normalization.
|
| |
|
| Submission date |
Jun 28, 2013 |
| Last update date |
Feb 28, 2014 |
| Contact name |
Chi-Cheng Huang |
| E-mail(s) |
[email protected]
|
| Organization name |
Cathay General Hospital SiJhih
|
| Department |
Department of Medical Education and Research
|
| Street address |
No.2, Lane 59, Jiancheng Rd. Xizhi Dist.
|
| City |
New Taipei City |
| ZIP/Postal code |
22174 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE48390 |
Concurrent Gene Signatures for Han Chinese Breast Cancers [Affymetrix] |
| GSE48391 |
Concurrent Gene Signatures for Han Chinese Breast Cancers |
|
