|
| Status |
Public on Jun 29, 2013 |
| Title |
subject B before exercise |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
serum_before exercise
|
| Organism |
Homo sapiens |
| Characteristics |
subject: healthy male
timepoint: before an acute resistance exercise
tissue: serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using QIAzol following manufacturer's instructions
|
| Label |
Hy3
|
| Label protocol |
miRCURY LNA microRNA Hi Power Labeling kit
|
| |
|
| Channel 2 |
| Source name |
Total RNA from the pooled serum
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pooled human serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using QIAzol following manufacturer's instructions
|
| Label |
Hy5
|
| Label protocol |
miRCURY LNA microRNA Hi Power Labeling kit
|
| |
|
| |
| Hybridization protocol |
Labeled RNA was hybridized to the microarray slide for 16-20 hr at 56 degrees C and washed with miRCURY LNA microRNA Power Labeling kit (Exiqon) according to manufacture's protocol.
|
| Scan protocol |
Hybridization signals were detected by DNA microarray scanner G2505B (Agilent Technologies) with resolution of 10 micrometres . The scanned images were analyzed using Agilent Feature Extraction ver. 9.1.3.1.
|
| Description |
B-pre
|
| Data processing |
At first Hy3 (samples) signal was normalized with division used Hy5 (Universal reference) signal. Then each signal value of unique probe ID was calculated from average of Hy3/Hy5 normalized signal intensities of replicated probes.
|
| |
|
| Submission date |
Jun 28, 2013 |
| Last update date |
Jun 29, 2013 |
| Contact name |
Takayuki Akimoto |
| E-mail(s) |
[email protected]
|
| Organization name |
The University of Tokyo
|
| Street address |
Hongo 7-3-1
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17382 |
| Series (1) |
| GSE48405 |
Human plasma: circulating microRNA after acute resistance exercise |
|
