|
| Status |
Public on Oct 28, 2014 |
| Title |
Lung of bleomycin-treated rat after 3D, replicate 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Lung_BLEO_3D_R4
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Lung
treatment: BLEOMYCIN
time: 3 DAYS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using MagMAX-96 for microarrays kit (Life technologies, Foster City CA, USA ) following the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
200 ng of totRNA were labeled using the Low Input Quick Amp Labeling kit (Agilent, Austin TX, USA) following the manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
Stratagene Reference
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Pool of 14 rat cell types
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using MagMAX-96 for microarrays kit (Life technologies, Foster City CA, USA ) following the manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
200 ng of totRNA were labeled using the Low Input Quick Amp Labeling kit (Agilent, Austin TX, USA) following the manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
a pool of 825 ng of Cy3 labeled aRNA (examined sample) and 825 ng of Cy5 labeled aRNA rat reference (Agilent, Austin TX, USA) were hybridized using the Agilent Hybridization kit (Agilent, Austin TX, USA) according to the manufacturer's instructions. Hybridization was performed for 17 hours and washed according to Agilent's instructions.
|
| Scan protocol |
Scanned on an Agilent G2505B scanner using Agilent scan control (version 7.0) Images were quantified using Agilent Feature Extraction Software (version 10.5.5.1).
|
| Data processing |
BioConductor package vsn was used for variance-stabilizing normalization and glog transformation
|
| |
|
| Submission date |
Jul 01, 2013 |
| Last update date |
Oct 29, 2014 |
| Contact name |
Axel Klenk |
| E-mail(s) |
[email protected]
|
| Organization name |
Idorsia Pharmaceuticals Ltd.
|
| Department |
Research Informatics
|
| Street address |
Hegenheimermattweg 91
|
| City |
Allschwil |
| ZIP/Postal code |
4123 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL7294 |
| Series (1) |
| GSE48455 |
Translation value of the bleomycin rat model for the treatment of patients with Idiopathic Pulmonary Fibrosis (IPF) |
|
