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Status
Public on Jun 01, 2007
Title
47r rna vs human ref. rna - repeat 2 - mAdbID:43757
Sample type
RNA
Channel 1 Source name
Universal Human Reference RNA - Cy3
Organism
Homo sapiens Characteristics
Other: Equal quantities of total RNA from each cell line (brain, breast, B-lymphocyte, cervix, liver, liposarcoma, macrophages, skin, testis, Y-lymphocyte) were pooled together.
Biomaterial provider
Stratagene (La Jolla, CA)
Extracted molecule
total RNA
Label
cy3
Label protocol
Cy3 Sample Labeling Protocol - generic
Channel 2 Source name
47r DNR- Cy5
Organism
Homo sapiens Characteristics
Strain: athymic nude mouse
Extracted molecule
total RNA
Extraction protocol
Sample Extraction Protocol
Label
cy5
Label protocol
Cy5 Sample Labeling Protocol - generic
Hybridization protocol
FairPlay Microarray Labeling Kit
Description
mAdb experiment ID: 43757
Data processing
After background correction and removal of flagged values, log base 2 expression ratios were median centered and linear transformed to obtain the log and linear values given in the data table.
Submission date
Jul 07, 2006
Last update date
Jul 10, 2006
Contact name
binwu tang
E-mail(s)
[email protected] Phone
3014968344
Fax
3014968395
Organization name
nih
Department
nci
Lab
lcrc
Street address
9000 rockville pike
City
bethesda
State/province
MD
ZIP/Postal code
20892
Country
USA
Platform ID
GPL1262 Series (1)
GSE5265 TGF-beta on breast cancer cell
Data table header descriptions ID_REF NCI mAdb well id plus replicate number
VALUE same as UNF_VALUE but with flagged values removed
PRE_VALUE Calibrated Ratio (CY5 channel/CY3 channel)
Slide_block Array block location
Slide_column Array column location
Slide_row Array row location
CY5_mean Red Channel Sample mean Signal (Background Subtracted)
CY5_SD Red Channel Sample Standard Deviation
CY5_BKD_median Red Channel Sample median Background Level
CY5_BKD_SD Red Channel Sample Background Standard Deviation
CY3_mean Green Channel Sample mean Signal (Background Subtracted)
CY3_SD Green Channel Sample Standard Deviation
CY3_BKD_median Green Channel Sample median Background Level
CY3_BKD_SD Green Channel Sample Background Standard Deviation
Flag Quality flag 0->good, -50->Not found, -100->Bad
UNF_VALUE log ratio (log2 of PRE_VALUE)
Data table ID_REF VALUE PRE_VALUE Slide_block Slide_column Slide_row CY5_mean CY5_SD CY5_BKD_median CY5_BKD_SD CY3_mean CY3_SD CY3_BKD_median CY3_BKD_SD Flag UNF_VALUE 159762_1
0.221
1.166
1
1
1
232
140
84
55
320
59
188
39
0
0.221
160338_1
-0.283
0.822
1
1
2
1916
626
86
65
2512
689
196
66
0
-0.283
160914_1
0.513
1.427
1
1
3
385
262
83
53
414
136
194
56
0
0.513
161490_1
1.869
3.653
1
1
4
4686
2629
84
51
1498
813
188
43
0
1.869
162066_1
-1.142
0.453
1
1
5
985
485
87
56
2253
787
192
54
0
-1.142
162642_1
-0.527
0.694
1
1
6
1752
767
88
49
2686
1112
193
47
0
-0.527
163218_1
1.246
2.372
1
1
7
1059
373
92
69
619
224
195
54
0
1.246
163794_1
-0.349
0.785
1
1
8
194
140
83
338
338
85
191
89
0
-0.349
164370_1
-1.136
0.455
1
1
9
89
43
82
53
205
53
189
45
-50
-1.136
164946_1
0.848
1.800
1
1
10
474
214
88
53
416
126
193
50
0
0.848
165522_1
-0.112
0.926
1
1
11
1237
550
87
63
1493
526
201
63
0
-0.112
166098_1
0.069
1.049
1
1
12
445
160
89
56
545
164
192
43
0
0.069
166674_1
0.003
1.002
1
1
13
1047
368
88
47
1187
362
192
56
0
0.003
167250_1
1.447
2.727
1
1
14
2192
4729
81
52
989
928
184
43
0
1.447
167826_1
-0.142
0.906
1
1
15
252
108
89
65
376
133
189
42
0
-0.142
168402_1
0.391
1.311
1
1
16
818
413
78
55
774
340
187
49
0
0.391
168978_1
1.156
2.228
1
1
17
104
68
89
80
200
45
193
55
-50
1.156
169554_1
0.532
1.446
1
1
18
2194
855
89
78
1711
454
197
71
0
0.532
159766_1
-0.343
0.788
1
2
1
1614
653
88
50
2207
1042
194
46
0
-0.343
160342_1
0.582
1.497
1
2
2
1239
372
89
64
993
224
194
54
0
0.582
9128 599 Kbytes .Supplementary data files not provided
