|
| Status |
Public on Aug 24, 2009 |
| Title |
Rat ventricle normal bio replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
normal rat ventricular tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: ventricle
gender: Male
strain: Sprague-Dawley
weight: 225-275 grams
|
| Treatment protocol |
Ventricular free wall was obtained from Sprague Dawley rats, sacrificed by decapitation. The tissue was snap-frozen in liquid nitrogen and stored at -80 ºC until RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 micrograms of total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
A total of 20 microg of fragmented cRNA was hybridized to each GeneChip® Rat Genome 230 2.0 array. After 16 hours of incubation at 45 C, the GeneChips® were washed and stained with Streptavidin- phycoerythrin biotinylated anti-streptavidin antibody in the Affymetrix GeneChip® Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® 3000 Scanner
|
| Description |
Gene expression data from rat ventricular free wall
|
| Data processing |
The data was analyzed with Robust Multiple-Array Averaging (RMA) which uses a model based background correction, quantile normalization and robust averaging expression summary method.
|
| |
|
| Submission date |
Jul 07, 2006 |
| Last update date |
Mar 29, 2010 |
| Contact name |
Adolfo J de Bold |
| E-mail(s) |
[email protected]
|
| Phone |
(613) 761-4265
|
| Fax |
(613) 761-1597
|
| Organization name |
University of Ottawa Heart Institute
|
| Department |
Cardiovascular Endocrinology
|
| Street address |
40 Ruskin St.
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1Y 4W7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE5266 |
Expression data from normal atria and ventricles |
|
