|
| Status |
Public on Jul 15, 2015 |
| Title |
lung wt chall rep3 |
| Sample type |
RNA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
Sex: female
strain: C57BL/6N
genotype/variation: wild type
treatment: challenged three time by inhalative exposure to OVA aerosol (1% in PBS) for 30 min
age: 17 weeks
mouse id: 30126536
|
| Treatment protocol |
Mice were sensitized by i.p. injection of 10 µg OVA in 2 mg alum in 200 µg PBS. Mice were subsequently challenged three time by inhalative exposure to OVA aerosol (1% in PBS) for 30 min for weeks after i.p. injection as described in Alessandrini et al, 2006, J Allergy Clin Immunol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using HiScan
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
|
| |
|
| Submission date |
Aug 09, 2013 |
| Last update date |
Jul 15, 2015 |
| Contact name |
Martin Irmler |
| Organization name |
Helmholtz Zentrum München GmbH
|
| Department |
Institute of Experimental Genetics
|
| Lab |
Gene Regulation & Epigenetics
|
| Street address |
Ingolstaedter Landstrasse 1
|
| City |
Neuherberg |
| State/province |
Bayern |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE49694 |
Transcriptome analysis of Cox4-2 knockout mice after OVA challenge |
|
