Line: Canton-S selected Gender: male Age: 5 days Tissue: head
Treatment protocol
Male flies were selected for aggressive behavior for 21 generations
Extracted molecule
total RNA
Extraction protocol
Trizol extraction
Label
biotin
Label protocol
Standard Affymetrix
Hybridization protocol
Standard Affymetrix
Scan protocol
Standard Affymetrix
Description
Males were frozen in groups of ~120 between 2:00 and 2:30 PM at 5 days of age, and were randomly collected from culture bottles and stored in groups of ~40