|
| Status |
Public on Aug 21, 2013 |
| Title |
EA_v24_6 |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
population: European-American
treatment: 1,25-dihydroxyvitamin D
in vitro lymphocyte vitd sensitivity (lgs - %inhibition by vitd): -48.98
duration of treatment (hours): 8
gender: male
age (years): 23.59
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy Plus mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
22
|
| Data processing |
quantile normalized and variance transformed using LUMI package in R
|
| |
|
| Submission date |
Aug 20, 2013 |
| Last update date |
Aug 21, 2013 |
| Contact name |
Joseph C Maranville |
| E-mail(s) |
[email protected]
|
| Phone |
7738345239
|
| Organization name |
920 E. 58th Street
|
| Street address |
920 E. 58th Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE50012 |
Comparison of cellular and transcriptional responses to 1,25-dihydroxyvitamin D3 and glucocorticoids in peripheral blood mononuclear cells |
|
