|
| Status |
Public on Jan 01, 2014 |
| Title |
H1299_shPTK7-1 |
| Sample type |
RNA |
| |
|
| Source name |
H1299_shPTK7
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-H1299
cell type: lung cancer cells
transfected with: shPTK7 (PTK7 knockdown)
|
| Treatment protocol |
Standard lentiviral production methods were used in 293FT cells. The shRNA lentivirus was used to infect the cell lines for 2 days, then subjected to 2 days of puromycin selection to ensure a pure population infected cells. RNA was collected at day 4 after infection.
|
| Growth protocol |
NCI-H1299 and NCI-H2009 were maintained in standard conditions of RPMI media supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol was used to extract RNA, followed by DNAse treatment in a Qiagen RNA column.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix biotinylation protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Standard Affymetrix
|
| Description |
H1299_shPTK7-1
|
| Data processing |
R (v.2.14.0; 2011-10-31) BiocInstaller (v.1.2.1), RMA
|
| |
|
| Submission date |
Aug 23, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Dedeepya Vaka |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford
|
| Department |
Pediatrics
|
| Lab |
Alejandro Sweet-Cordero
|
| Street address |
265 Campus Drive
|
| City |
Palo Alto |
| ZIP/Postal code |
94035 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE50138 |
Lentiviral shRNA knockdown of PTK7 in lung adenocarcinoma cell lines NCI-H1299 and NCI-H2009 |
|
