|
| Status |
Public on Dec 04, 2014 |
| Title |
21 days pregnancy, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
liver at 21 days of pregnancy
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: CYP2D6 transgenic
tissue: liver
stage of pregnancy: 21 days of pregnancy
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
250 ng of total RNA was processed for biotin-labeled target preparation according to the GeneChip 3' IVT Express protocol (Affymetrix, Inc).
|
| |
|
| Hybridization protocol |
12 µg of biotin-labeled and fragmented target cRNA was hybridized to MG 430 2.0 expression array for 16 h at 45°C and 60 rpm in an Affymetrix Hybridization Oven 640. After hybridization, arrays were washed and stained on a GeneChip Fluidics Station 450 (Affymetrix, Inc.).
|
| Scan protocol |
Arrays were scanned using the GeneChip Scanner 3000 7G to extract CEL files. Intensity expression data by GeneChip Operating Software v. 1.4 (MicroArray Suite 5.0).
|
| Description |
CYP2D6-humanized mouse livers. Transgenic mice harboring CYP2D6 (PMID:16033950).
|
| Data processing |
The raw data were then summarized with RMA and quantile normalization using Affymetrix Power Tools.
|
| |
|
| Submission date |
Aug 23, 2013 |
| Last update date |
Dec 04, 2014 |
| Contact name |
Wei Zhang |
| E-mail(s) |
[email protected]
|
| Phone |
312-413-2024
|
| Organization name |
University of Illinois
|
| Department |
Pediatrics & Institute of Human Genetics
|
| Lab |
Zhang Lab
|
| Street address |
840 S. Wood Street
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE50166 |
Effects of pregnancy on hepatic gene expression in CYP2D6-humanized mice |
|
