|
| Status |
Public on Jul 30, 2014 |
| Title |
WT (clone 3) |
| Sample type |
RNA |
| |
|
| Source name |
Stable clone of NIH3T3 cell line, wild type PreS/S gene of HBV, clone 3.
|
| Organism |
Mus musculus |
| Characteristics |
cell line: NIH3T3
treatment: Stable transfectant of plasmid encoding wild type PreS/S gene of HBV.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with QIAGEN RNeasy mini kit according to protocol provided in the kit by the manufacturer. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the TargetAmp Nano-G Bioti- aRNA Labeling kit (Epicentre) for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
WT-3
|
| Data processing |
Quantile normalization using GeneSpring11.
|
| |
|
| Submission date |
Aug 26, 2013 |
| Last update date |
Jul 30, 2014 |
| Contact name |
Shih Sheng Jiang |
| E-mail(s) |
[email protected]
|
| Organization name |
National Health Research Institutes
|
| Department |
National Institute of Cancer Research
|
| Street address |
No. 35 Keyan Road, Zhunan County
|
| City |
Miaoli County |
| ZIP/Postal code |
350 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE50180 |
Gene expression profiles of NIH3T3 cells stably transfected with the plasmids encoding the wild type PreS/S gene or the nonsense mutant gene (sW182*) of HBV. |
|
