|
| Status |
Public on Sep 23, 2013 |
| Title |
Knockout (KO) 10 wk Mat rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MED1 KO_10wk_maturation
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: MED1 fl/fl K14 driven Cre
age: 10 weeks
phenotype: Incisors generate hairs but lack enamel
tissues: microdissected dental tissues from lower incisors
Stage: maturation stage (Mat)
|
| Treatment protocol |
Dental tissues generating hairs were dissected from MED1 KO mouse lower incisors, and the tissues from 3 mice were combined, then RNA extracted. Equivalent tissues in control littermate mice were also dissected.
|
| Growth protocol |
Coactivator MED1 is removed from keratin 14 (K14) expressing epithelia by using Crelox stragety
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from dissected dental tissues by using Pico pure RNA purification kit (ABI)
|
| Label |
biotin
|
| Label protocol |
Standard Illumina hybridization protocol; cDNA is labeled by biotin and hybridized with Cy3-streptoavidin
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol done by UCLA neuroscience genomics core
|
| Scan protocol |
Standard Illumina hybridication protocol done by UCLA neuroscience genomics core
|
| Description |
KO1
|
| Data processing |
data were normalized by using quantile method in Genome studio (v.V2011.1; Module Gene Expression v.1.9.0).
|
| |
|
| Submission date |
Aug 30, 2013 |
| Last update date |
Sep 23, 2013 |
| Contact name |
Yuko Oda |
| E-mail(s) |
[email protected]
|
| Phone |
4152692370
|
| Organization name |
VA Medical Center UCSF
|
| Department |
Endocrine Research
|
| Street address |
111N-MB 360 1700 Owens St, Suite 360
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE50502 |
Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair [maturation stage] |
| GSE50504 |
Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair |
|
