|
| Status |
Public on Sep 06, 2013 |
| Title |
activated WT T-cells day 4 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Balb/c mouse WT1
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD3+ T-cells
genotype: WT
age: 12 wk
genetic background: Balb/C
gender: Male
|
| Treatment protocol |
Isolated cells were cultured on tissue culture plates with 3 mg/ml of plate-coated anti-mouse CD3 antibody and 3 mg//ml of soluble anti-mouse CD28 antibody for 4 days.
|
| Growth protocol |
Isolated CD3+ T-cells were cultured in RPMI supplemented with 10% FBS, 100 U/mL penicillin/streptomycin, and 55 mM 2-mercaptoethanol at 37°C in 95% humidity and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Kit from Qiagen. We followed company's instruction.
|
| Label |
biotin
|
| Label protocol |
100 ng/ml total RNA was reverse transcribed with T7 oligo(dT) primer and amplified. After amplification, multiple copies of biotin-modified aRNA was generated by using Affymetrix GeneChip 3' IVT Express Kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
All prepared samples were labeled and hybridized to Affymetrix mouse Gene ST 1.0 GeneChip.
|
| Scan protocol |
Affymetrix GeneChip scanner 3000 was used for scanning and Affymetrix GCOS software was used for data collection.
|
| Description |
Gene expression data from WT Balb/C T-cells
|
| Data processing |
RMA
|
| |
|
| Submission date |
Sep 05, 2013 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Seok-Joo Kwon |
| Organization name |
NCI/NIH
|
| Street address |
37 Convent Dr.
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE50617 |
Expression data from mouse CD3+ T-cells from WT and KLF13 KO mice |
|
