|
| Status |
Public on Aug 19, 2014 |
| Title |
Human gastric cancerous tissue3 |
| Sample type |
RNA |
| |
|
| Source name |
Human gastric cancerous tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 48
tissue: gastric cancerous tissue
|
| Treatment protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Growth protocol |
the samples were stored in -80 degrees prior to extract preparation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre)
|
| Label |
Cy3
|
| Label protocol |
1 µg of total RNA were primed with 1.2 μL of T7 Promoter Primer at 65°C for 10 min, then prepare labeling reaction at 40°C for 2 h in the presence of 8.5 μL of cDNA Master Mix, Add 60 μL of Transcription Master Mix to each sample tube. Gently mix by pipetting. Incubate samples at 40°C for 2 hours. And then purify the labeled/amplified RNA and labeled cRNA QC, finally do the hybridaztion.
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the Human LncRNA Array v2.0 (8 x 60K, Arraystar).
|
| Scan protocol |
After having washed the slides, the arrays were scanned by the Agilent Scanner G2505C.Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images.
|
| Description |
Gene expression of gastric cancerous tissue
|
| Data processing |
Quantile normalization and subsequent data processing were performed using the GeneSpring GX v11.5.1 software package (Agilent Technologies)
|
| |
|
| Submission date |
Sep 09, 2013 |
| Last update date |
Aug 20, 2014 |
| Contact name |
Ye Hu |
| E-mail(s) |
[email protected]
|
| Organization name |
Division of Gastroenterology and Hepatology, Shanghai Jiao-Tong University School of Medicine Renji Hospital
|
| Street address |
145 Middle Shandong Rd
|
| City |
Shanghai |
| ZIP/Postal code |
200001 |
| Country |
China |
| |
|
| Platform ID |
GPL13825 |
| Series (1) |
| GSE50710 |
LncRNA/mRNA expression profiling for 20 human samples |
|
