|
| Status |
Public on Apr 11, 2014 |
| Title |
SF539 scramble siRNA replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
SF539 GBM cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SF539
transfection: Scramble siRNA
|
| Treatment protocol |
siRNAs were transfected into cells in antibiotic-free medium using Lipofectamine RNAiMAX , medium was changed after 4–6 h and cells were harvested after 48 hours .
|
| Growth protocol |
SNB19 cells were cultured in DMEM media supplemented with 10% FBS and penicillin-streptomycin at 37 °C in 5% CO2. SF539 cells were grown in MEM media with 10% FBX and penicillin-streptomycin at 37 °C in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using Rneasy Plus Mini Kit (Qiagen). RNA quality was determined with the Nanodrop Spectrophotometer (Thermo).
|
| Label |
Biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Data processing |
data was normalized and analyzed using partek software and R packages. Specifically log2 transformation was used for data normalization.
|
| |
|
| Submission date |
Sep 13, 2013 |
| Last update date |
Apr 12, 2014 |
| Contact name |
Yongxing Gong |
| E-mail(s) |
[email protected]
|
| Phone |
6468882783
|
| Organization name |
Momerial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE50864 |
PARK2 knockdown in 2 glioma cell lines |
|
