|
| Status |
Public on Sep 05, 2006 |
| Title |
human nonfailing LV myocardium, NonFailing2 |
| Sample type |
RNA |
| |
|
| Source name |
unused donor heart with normal LV function
|
| Organism |
Homo sapiens |
| Characteristics |
normally functioning myocardium from unused donor heart
|
| Treatment protocol |
explanted myocardium snap frozen in liquid nitrogen
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation cRNA were hybridized with HU133A arrays according to Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from nonfailing unused donor heart
|
| Data processing |
Data were normalized using Robust Multi-Array Analysis (RMA). www.bioconductor.org
|
| |
|
| Submission date |
Jul 28, 2006 |
| Last update date |
Sep 05, 2006 |
| Contact name |
Thomas Cappola |
| E-mail(s) |
[email protected]
|
| Organization name |
Univeristy of Pennsylvania School of Medicine
|
| Department |
Penn Cardiovascular Institute
|
| Street address |
6 Penn Tower, 3400 Spruce Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE5406 |
Human ischemic cardiomyopathy, idiopathic cardiomyopathy, and nonfailing controls |
|
