|
| Status |
Public on Sep 19, 2016 |
| Title |
prostate_cancer_primary_BCR_83 |
| Sample type |
RNA |
| |
|
| Source name |
primary prostate cancer FFPE tissue sample, did not develop metastasis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary prostate cancer
metastatic event: 0
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Not applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from four 10 um FFPE sections. The highest Gleason grade was macrodissected from surrounding non-neoplastic cells, guided by an H&E stained slide for tumor identification. RNA was extracted and purified using the Roche HighPure FFPE kit.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, fragmented and biotinylated using the WT-Ovation FFPE system (NuGen, San Carlos, CA) according to the manufacturer's recommendations with minor modifications.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials and protocols.
|
| Scan protocol |
Affymetrix GeneChIP Scanner 3000 7G.
|
| Description |
GBX.VAL.PCA83
Samples are from men with biochemical recurrence (BCR) following radical prostatectomy (RP).
|
| Data processing |
Data were processed using fRMA software package (Bioconductor).
Probe group file: HuEx-1_0-st-v1.r2.pgf
Meta-probeset file: Not applicable.
|
| |
|
| Submission date |
Sep 20, 2013 |
| Last update date |
Sep 19, 2016 |
| Contact name |
Elai Davicioni |
| E-mail(s) |
[email protected]
|
| Organization name |
Veracyte, Inc
|
| Street address |
9725 Lusk Blvd
|
| City |
San Diego |
| State/province |
California |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE51066 |
A genomic classifier predicting metastatic disease progression in men with biochemical recurrence after prostatectomy |
|
