|
| Status |
Public on Sep 24, 2013 |
| Title |
MCF10A_GFP_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF10A GFP control, day 4
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
genotype/variation: GFP control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were prepared according to the standard Affymetrix protocol from 8 ug total RNA.
|
| |
|
| Hybridization protocol |
Samples were hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays according to the standard Affymetrix protocol.
|
| Scan protocol |
Scanned with GeneChip scanner 3000 7G.
|
| Description |
GFP.1
|
| Data processing |
The data was pre-processed using the RMA algorithm from the affy (v1.30.0) package for R (v2.13.2) and updated Entrez Gene annotations (hgu133plus2hsentrezgcdf_14.0.0).
|
| |
|
| Submission date |
Sep 24, 2013 |
| Last update date |
Sep 24, 2013 |
| Contact name |
Michelle Chan-Seng-Yue |
| E-mail(s) |
[email protected]
|
| Organization name |
Ontario Institute for Cancer Research
|
| Street address |
101 College Street
|
| City |
Toronto |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16311 |
| Series (1) |
| GSE51123 |
3D Expression Data of Myc and Myc phospho-mutants |
|
