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Sample GSM124480 Query DataSets for GSM124480
Status Public on Aug 02, 2006
Title embryo at 0-2hour, fraction 9, biological rep2
Sample type RNA
 
Source name Drosophila embryos
Organism Drosophila melanogaster
Characteristics Canon S embryos
Treatment protocol embryos were collected in 2-hour intervals and aged to generate animals 0-2, 4-6 and 8-10 hour old.
Extracted molecule total RNA
Extraction protocol the dechorionated embryos were first incubated with 0.1mg/ml cycloheximide in PBS for 10 minutes on ice, then homogenized with a motorized plastic pellet pestle in a lysis buffer (20mM Tris-HCl, pH7.4, 140mM Kcl, 5mM MgCl2, 0.5mM DTT, 1% Triton X-100, 0.1mg/ml cycloheximide, 1mg/ml heparin, 50unit/ml RNasin) and incubated for 10 minutes on ice. The nuclei and debris were removed by centrifugation at 12,000x g for 10min at 4oC, and supernatants was loaded onto 20-50% sucrose gradients with the same extraction buffer without Triton x-100.
Label biotin
 
Hybridization protocol Following fragmentation, at least 10 microg of cRNA were hybridized on GeneChip Drosophila Genome 2.0 Array.
Description Gene expression data from embryos during first 10 hours after egg laying to see the global and transcript-specific translation profile
Data processing Gene expression measures were computed using the Robust Multichip Average (RMA) method and implemented in the Bioconductor R package.
 
Submission date Aug 01, 2006
Last update date Aug 28, 2018
Contact name Soyeon Ahn
E-mail(s) [email protected]
Organization name UC Berkeley
Street address 367 Evans Hall #3860
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platform ID GPL1322
Series (1)
GSE5430 Expression data from early Drosophila embryo
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE intensity after RMA

Data table
ID_REF VALUE
1616608_a_at 10.03076499
1622892_s_at 9.626845969
1622893_at 3.205744675
1622894_at 4.666462425
1622895_at 9.151892525
1622896_at 5.102793732
1622897_at 6.101912416
1622898_a_at 10.46500571
1622899_at 4.459600481
1622900_at 3.271426582
1622901_at 4.058690614
1622902_at 4.882308897
1622903_s_at 9.821554635
1622904_at 4.772329998
1622905_at 3.702237572
1622906_at 7.215028015
1622907_at 8.912044361
1622908_a_at 6.841328489
1622909_at 7.521753111
1622910_at 3.429143355

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM124480.CEL.gz 3.2 Mb (ftp)(http) CEL

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