|
| Status |
Public on Oct 22, 2013 |
| Title |
PBMCs rep13 |
| Sample type |
RNA |
| |
|
| Source name |
Baboon Peripheral blood mononuclear cells
|
| Organism |
Papio hamadryas |
| Characteristics |
gender: female
age (yrs): 20.18
tissue: Baboon Peripheral blood mononuclear cells
|
| Treatment protocol |
Blood was placed in an 8-mL BD Vacutainer® CPT™ Tube system for collection of PBMCs. After centrifugation, the cell layer was collected and cells were washed according to the manufacturer’s protocol. The cells were then resuspended in RPMI-C medium (10% DMSO, 90% RPMI 1640, Fisher Scientific, Pittsburgh, PA) and stored at -80°C. PBMC RNA was obtained using the QIAGEN RNeasy Kit.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the resulting powdered bone using the QIAGEN RNeasy Lipid Tissue Kit (Qiagen Corp. Inc., Valencia, CA) according to manufacturer’s instructions. PBMC RNA was obtained using the QIAGEN RNeasy Kit. All RNA was treated using the Qiagen RNase-free DNase set.
|
| Label |
biotin
|
| Label protocol |
Isolated total RNA was then used to generate anti-sense RNA using the Ambion MessageAmp II Amplification Kit (Ambion, Austin, TX) followed by RNA biotinylation and amplification with the Illumina Total Prep RNA Amplification kit (Illumina Inc., San Diego, CA).
|
| |
|
| Hybridization protocol |
Hybridization was performed according to manufacturer's instructions.
|
| Scan protocol |
Samples were scanned on the Illumina BeadArray 500GX Reader using Illumina BeadScan image data acquisition software (version 2.3.0.13).
|
| Description |
4273735038_D
|
| Data processing |
Raw data were uploaded to Genome Studio (Illumina Inc., San Diego, CA), quantile normalized, and background corrected. Differential gene expression was analyzed using Genome Studio to compare expression of genes between the two tissues and reported as a Diff Score.
|
| |
|
| Submission date |
Oct 21, 2013 |
| Last update date |
Oct 22, 2013 |
| Contact name |
Heather B Coan |
| E-mail(s) |
[email protected]
|
| Phone |
8282273665
|
| Organization name |
Western Carolina University
|
| Department |
Biology Department
|
| Street address |
132 Natural Science Building
|
| City |
Cullowhee |
| State/province |
NC |
| ZIP/Postal code |
28723 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE51495 |
Peripheral blood mononuclear cell- and cortical bone-derived transcriptional profiles |
|
