|
| Status |
Public on Aug 11, 2014 |
| Title |
gastrocnemius hindlimb muscle, smoke&hypoxia-exposed, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
gastrocnemius hindlimb muscle_smoke&hypoxia-exposed
|
| Organism |
Cavia porcellus |
| Characteristics |
strain: Hartley guinea pigs
gender: male
condition: smoke&hypoxia-exposed
tissue: gastrocnemius hindlimb muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini extraction kits (Qiagen, USA) according to the manufacturer’s recommendations
|
| Label |
Cy3
|
| Label protocol |
One hundred nanograms of total RNA from each sample was amplified and converted into labelled cRNA using Agilent’s Low Input Quick Amp Labelling Kit according to the manufacturer’s recommendations
|
| |
|
| Hybridization protocol |
Cy3-labelled cRNA (600 ng/sample) was hybridized at 65C for 16 hours to our custom Cavia porcellus oligonucleotide microarray (manufactured by Agilent) in randomized sample order
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60k array slides (Scan Area 61x21.6 mm, Scan resolution 5um).
|
| Description |
US92200305_254066610006_S01_GE1_107_Sep09_1_3.txt
|
| Data processing |
The data were analyzed with R/Bioconductor (v3.0.1), quantile normalized values were obtained with the 'marray' package.
|
| |
|
| Submission date |
Oct 23, 2013 |
| Last update date |
Aug 11, 2014 |
| Contact name |
Peter Davidsen |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Liverpool
|
| Street address |
Crown Street
|
| City |
Liverpool |
| State/province |
Merseyside |
| ZIP/Postal code |
L69 7ZB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17826 |
| Series (2) |
| GSE51601 |
Global gene expression profiling of guinea pig whole-lung and hindlimb muscles following long-term exposure to cigarette smoke and/or hypoxia |
| GSE56099 |
A systems biology approach reveals a link between systemic cytokines and skeletal muscle energy metabolism in a rodent smoking model and human COPD |
|
